Image based High Content Analysis can support all signaling pathway assays which rely on the translocation of a fluorescently labeled signaling molecule. Transcription factor activation, for example, can be monitored based on their translocation from cytosol to nucleus. Many other signaling molecules translocate from the cytosol to receptor complexes at the plasma membrane.

Akt3-GFP in untreated cells (left) and in activated cells (right).