Cell differentiation is a multi-step procedure associated with the expression of a specific set of cellular markers in each step, for example the progression of a pluripotent stem cell to a progenitor cell and a fulCell differentiation is a multi-step procedure associated with the expression of a specific set of cellular markers in each step, for example the progression of a pluripotent stem cell to a progenitor cell and a fully differentiated tissue specific cell type. The morphology of the cell also changes during differentiation.ly differentiated tissue specific cell type. The morphology of the cell also changes during differentiation.

Classification of GFAP positive (green squares), Tuj1 positive (red squares) and GFAP/Tuj1 positive cells (green and red squares) in a mixed population of neuronal and Glia cells.