This application note outlines a new workflow with one instrument platform (LC/MS/MS) method that is ideal for simultaneous analysis of pesticide and mycotoxins residue in cannabis. This application note demonstrates the performance of a LC/MS/MS ESI and APCI method with a single solvent extraction step to meet Florida regulations for 67 pesticides and 5 mycotoxins in complex cannabis flower matrix in place as of the date of this publication.